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Humoral immune profile after intradermal immunization of BALB/c mice with neoglycocomplexes. A–D) Allergen-specific IgG two weeks after the last immunization was analyzed via ELISA. Two individual experiments are shown (experiment 1 — A and B, experiment 2 — C–F). E and F) Biologically functional IgE of experiment 2 was assessed by RBL assay. Serum dilutions were 1:100 for IgG and 1:17 for IgE. Data are shown as individual data points (n = 3–5). * P < 0.05; ** P < 0.01; *** P < 0.001.
