Table 3.
Abiotic stress assay based on growth parameters of one-month old T1 seedlings of SAM lines
| Abiotic stress | UT | SAM3 | SAM8 | SAM17 |
|---|---|---|---|---|
| Without stress | ||||
| Shoot length (cm) | 12.316 ± 1.10 (100) | 8.750 ± 0.333 (71)* | NT | 7.983 ± 0.671 (65)a |
| Fresh weight (g) | 0.213 ± 0.03 (100) | 0.156 ± 0.016 (74) | NT | 0.126 ± 0.019 (60)a |
| Dry weight (g) | 0.0282 ± 0.01 (100) | 0.016 ± 0.002 (57) | NT | 0.009 ± 0.001 (4) |
| Salt | ||||
| (150 mM NaCl) | ||||
| Shoot length (cm) | 7.400 ± 0.288 (100) | 8.800 ± 0.352 (119)a | 10.280 ± 1.312 (138)a | 11.830 ± 0.520 (160)a |
| Fresh weight (g) | 0.238 ± 0.050 (100) | 0.600 ± 0.063 (252)a | 0.665 ± 0.193 (279)a | 1.83 ± 0.080 (768)a |
| Dry weight (g) | 0.010 ± 0.002 (100) | 0.041 ± 0.006 (410)a | 0.045 ± 0.013 (450)a | 0.097 ± 0.020 (970)a |
| (200 mM NaCl) | ||||
| Shoot length (cm) | NA | 6.333 ± 0.258 | NA | NA |
| Fresh weight (g) | NA | 0.553 ± 0.069 | NA | NA |
| Dry weight (g) | NA | 0.038 ± 0.005 | NA | NA |
| Drought | ||||
| (10 % PEG) | ||||
| Shoot length (cm) | 10.00 ± 0.417 (100) | 14.81 ± 1.161 (148)a | 14.44 ± 0.570 (144)a | 14.040 ± 0.863 (140)a |
| Fresh weight (g) | 0.467 ± 0.054 (100) | 2.174 ± 0.284 (465)a | 1.324 ± 0.200 (283)a | 1.704 ± 0.145 (364)a |
| Dry weight (g) | 0.040 ± 0.006 (100) | 0.1684 ± 0.037 (419)a | 0.112 ± 0.015 (279)a | 0.096 ± 0.027 (240) |
| (15 % PEG) | ||||
| Shoot length (cm) | NA | 11.06 ± 1.049 | 10.96 ± 1.01 | 11.56 ± 0.385 |
| Fresh weight (g) | NA | 1.0408 ± 0.218 | 0.789 ± 0.157 | 0.90 ± 0.07 |
| Dry weight (g) | NA | 0.0826 ± 0.021 | 0.058 ± 0.016 | 0.10 ± 0.016 |
| High temperature | ||||
| (40 °C) | ||||
| Shoot length (cm) | 10.566 ± 0.77 (100) | 9.860 ± 0.460 (94) | NT | 9.133 ± 0.251 (87) |
| Fresh weight (g) | 0.235 ± 0.03 (100) | 0.223 ± 0.033 (96) | NT | 0.183 ± 0.012 (78) |
| Dry weight (g) | 0.012 ± 0.01 (100) | 0.0270 ± 0.007 (225)a | NT | 0.010 ± 0.002 (84) |
| (45 °C) | ||||
| Shoot length (cm) | 5.283 ± 0.37 (100) | 8.183 ± 0.574 (155)a | NT | 8.066 ± 0.503 (153)a |
| Fresh weight (g) | 0.061 ± 0.01 (100) | 0.165 ± 0.014 (270)a | NT | 0.195 ± 0.015 (320)a |
| Dry weight (g) | 0.004 ± 0.00 (100) | 0.013 ± 0.000 (325)a | NT | 0.024 ± 0.002 (600)a |
| Low temperature | ||||
| (4–8 °C) | ||||
| Shoot length (cm) | 7.860 ± 0.385 (100) | 9.280 ± 0.475 (118)a | 8.680 ± 0.576 (110) | 8.960 ± 0.110 (114)a |
| Fresh weight (g) | 0.200 ± 0.03 (100) | 0.180 ± 0.019 (90) | 0.448 ± 0.075 (224)a | 0.265 ± 0.018 (132)a |
| Dry weight (g) | 0.008 ± 0.001 (100) | 0.022 ± 0.007 (275)a | 0.014 ± 0.002 (175)a | 0.013 ± 0.001 (175)a |
adenotes significant difference from respective control at 5 % level. NT not tested, NA not available due to lethality