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. 2011 Jul 8;17(3):271–280. doi: 10.1007/s12298-011-0074-6

Table 2.

Effect of different concentrations of cytokinins, benzylaminopurine (BAP) and thidiazuron (TDZ), supplemented individually and in combination with indole acetic acid (IAA) to woody plant basal medium, on per cent response and shoot organogenesis

PGR Per cent bud break (after 21 days) Number of shoot buds per explants (after 21 days) Number of shoots per explant (after 60 days) Length of the shoots (after 60 days) Comments
BAP TDZ IAA
0.5 90 ± 6.88ab 2.15 ± 0.109de 2.9 ± 0.32d 2.38 ± 0.12a y
1.0 100 ± 0a 3.25 ± 0.239d 4.85 ± 0.49c 2.42 ± 0.13a y
1.5 95 ± 5ab 1.3 ± 0.105e 3.65 ± 0.26cd 1.98 ± 0.126b y
0.5 90 ± 6.88ab 11.85 ± 0.36a 4.15 ± 0.39cd 1.53 ± 0.121c x
1.0 80 ± 9.17ab 11.2 ± 0.66ab 1.2 ± 0.09ef 1.043 ± 0.059d x
1.5 85 ± 8.19ab 9.9 ± 0.42b 0.35 ± 0.109f 0.72 ± 0.066e x
1.0 0.5 100 ± 0a 6.4 ± 0.49c 13.9 ± 0.68a 2.47 ± 0.072a y
1.0 1.0 95 ± 5ab 5.35 ± 0.109c 10.35 ± 0.74b 2.306 ± 0.191a y
0.5 0.5 90 ± 6.88ab 11.65 ± 0.949a 3.2 ± 0.46d 1.245 ± 0.102cd x
0.5 1.0 75 ± 9.93b 10.4 ± 0.81ab 1.65 ± 0.209e 0.576 ± 0.04e x

Data are means from 20 replicates ± SE and those representing similar letter in the appropriate column are not significantly different (ANOVA, P ≤ 0.05)

xThick, hyperhydric and stunted shoots

ySlender shoots with small diameter and exhibited pre-mature leaf fall