Table 2.
PGR | Per cent bud break (after 21 days) | Number of shoot buds per explants (after 21 days) | Number of shoots per explant (after 60 days) | Length of the shoots (after 60 days) | Comments | ||
---|---|---|---|---|---|---|---|
BAP | TDZ | IAA | |||||
0.5 | – | – | 90 ± 6.88ab | 2.15 ± 0.109de | 2.9 ± 0.32d | 2.38 ± 0.12a | y |
1.0 | – | – | 100 ± 0a | 3.25 ± 0.239d | 4.85 ± 0.49c | 2.42 ± 0.13a | y |
1.5 | – | – | 95 ± 5ab | 1.3 ± 0.105e | 3.65 ± 0.26cd | 1.98 ± 0.126b | y |
– | 0.5 | – | 90 ± 6.88ab | 11.85 ± 0.36a | 4.15 ± 0.39cd | 1.53 ± 0.121c | x |
– | 1.0 | – | 80 ± 9.17ab | 11.2 ± 0.66ab | 1.2 ± 0.09ef | 1.043 ± 0.059d | x |
– | 1.5 | – | 85 ± 8.19ab | 9.9 ± 0.42b | 0.35 ± 0.109f | 0.72 ± 0.066e | x |
1.0 | – | 0.5 | 100 ± 0a | 6.4 ± 0.49c | 13.9 ± 0.68a | 2.47 ± 0.072a | y |
1.0 | – | 1.0 | 95 ± 5ab | 5.35 ± 0.109c | 10.35 ± 0.74b | 2.306 ± 0.191a | y |
– | 0.5 | 0.5 | 90 ± 6.88ab | 11.65 ± 0.949a | 3.2 ± 0.46d | 1.245 ± 0.102cd | x |
– | 0.5 | 1.0 | 75 ± 9.93b | 10.4 ± 0.81ab | 1.65 ± 0.209e | 0.576 ± 0.04e | x |
Data are means from 20 replicates ± SE and those representing similar letter in the appropriate column are not significantly different (ANOVA, P ≤ 0.05)
xThick, hyperhydric and stunted shoots
ySlender shoots with small diameter and exhibited pre-mature leaf fall