Table 3.

Effect of glutamine, ADS and phlouroglucinol, supplemented in different concentrations and combinations to WPM having 1.0 BAP along with 0.5 mg/l IAA on shoot multiplication, as observed after 45 days of culture

Glutamine	ADS	PG	Number of shoots	Length of shoots (cm)	Comments
–	–	–	13.75 ± 0.421h	2.46 ± 0.07def	−a
50	–	–	12.89 ± 0.272hi	2.365 ± 0.11f	−
100	–	–	13.58 ± 0.27h	2.385 ± 0.13ef	+
200	–	–	12.5 ± 0.29i	2.63 ± 0.067cdef	+++
400	–	–	13.39 ± 0.31hi	2.607 ± 0.13cdef	−
200	50	–	17.7 ± 0.48e	2.58 ± 0.07cdef	++
200	100	–	21.72 ± 0.37c	2.8 ± 0.129cd	++
200	150	–	32.5 ± 0.35b	3.3 ± 0.11b	+++
200	200	–	13.33 ± 0.20hi	2.67 ± 0.108cdef	++
200	–	50	16.67 ± 0.24f	2.7 ± 0.10cde	++
200	–	100	19.29 ± 0.16d	3.3 ± 0.14b	++
200	–	200	15.05 ± 0.30g	2.87 ± 0.107c	++
200	150	50	21.6 ± 0.365c	2.71 ± 0.07cde	+++
200	150	100	35.65 ± 0.27a	3.74 ± 0.04a	++++

Values represent means ± SE of 20 replicates, those representing similar letter in the appropriate column are not significantly different (ANOVA, P < 0.05)

^aControl, from Table 2

− Leaf fall

+ Leaf fall at low frequency

++ No leaf fall

+++ No leaf fall, healthy shoots with increased girth

++++ No leaf fall, increased vigour of shoots