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. 2011 Jul 1;17(3):263–270. doi: 10.1007/s12298-011-0073-7

Table 3.

Effect of different carbohydrate sources and its concentrations on multiple shoot induction using nodal explants of B. orellana cultured in 5 μM 2-iP supplemented MS medium

Carbon source Conc. (mM) % response No. of shoots Shoot length Callus development
Glucose 43.3 73.5cd 10.76e 2.14d +
87.6 67.7cde 8.16f 2.56c ++
131.2 60.0de 4.30i 3.50b
Fructose 43.3 43.3f 5.23hi 2.66c
87.6 53.3ef 6.20g 1.63e
131.2 70.4cd 12.30d 1.10f
Sucrose 43.3 76.8bc 14.66c 2.96c
87.6 93.3a 35.71a 5.96a
131.2 87.0b 19.40b 3.56b +
Treatment Df (n-1) 8 9.55*** 537.59*** 106.78***
Carbon source (S) 2 27.04*** 1329.05*** 230.34***
Df (n-1)
Carbon conc.(C) 2 2.24 187.11*** 29.77***
Df (n-1)
S X C. Df (n-1) 8 9.55*** 537.59*** 106.78***

aMeans with in a column followed by same letters are not significantly different as determined by DNMRT (P < 0.05). ***significant at P < 0.001 level