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. 2012 Feb 27;32(3):318–326. doi: 10.1038/onc.2012.53

Figure 1.

Figure 1

Generation of Pik3cae20H1047R conditionally activatable knock-in allele. (a–d) Genomic locus encoding Pik3ca locus (a); targeting construct (b); targeted allele (c); targeted locus in the ES after removal of the neomycin cassette (d); and Pik3cae20H1047R allele following Cre-mediate activation (e) are shown. (f, g) Southern blotting using a 5′ probe (f) and a 3′ probe (g) was used to identify the appropriately targeted knock-in (ki) and wild-type (wt) allele. (h–i) Sanger sequencing of the cDNA obtained from Pik3cae20H1047R/+ mammary gland (h) and kidney (i) following Cre-mediated activation confirms the expression of the Pik3cae20H1047R allele in the mammary gland.