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. 2012 Feb 27;32(3):318–326. doi: 10.1038/onc.2012.53

Figure 2.

Figure 2

Pik3cae20H1047R/+ mice develop mammary tumors. (a, b) Mouse bearing tumor in the abdominal mammary (a) and thoracic mammary (b) glands. The white arrowheads indicate the location of the tumors. (c) Kaplan–Meier plot depicting tumor-free survival of two independent Pik3cae20H1047R lines following MMTV-Cre-mediated activation of the latent Pik3cae20mwt allele. Animals bearing mammary tumors (86.4% of line 1 and 84.2% of line 2 Pik3cae20H1047R/+ female mice had developed tumors) at the end point are shown in the plot. Two control groups, MMTV-Cre mice and Pik3cae20mwt, were tumor-free for the duration of the study. (d–g) Histology of Pik3cae20H1047R/+ mammary tumors. Analysis of H&E-stained tumors sections show the presence of multiple histological types, which include fibroadenoma (d), adenosquamous carcinoma (e), adenocarcinoma (f) and spindle cell tumor (g). (h) Western blot of Pik3cae20H1047R/+ mammary tumors representative of the histological types shows elevated pAKT levels (lanes 3–10) and pS6 (lanes 3–10) in all tumor types compared with control mammary glands (lanes 1–2). Tumors 9 and 10 were derived from passaging a primary spindle tumor in the SCID mice mammary fat pad.