Table 2.
Effect of plant growth regulators on shoot regeneration from leaf explant derived callus tissue of W. somnifera
| Growth regulators (mg/l) | % of shoot response | Mean number of shoots | Response initiation (in days) | |
|---|---|---|---|---|
| BAP | IBA | |||
| – | – | 15.0 ± 5.77d | 3.4 ± 0.4dc | 25 |
| 0.5 | – | 6.66 ± 1.66d | 2.4 ± 0.5e | 25 |
| 1.0 | – | 46.67 ± 4.4c | 6.0 ± 0.44c | 20 |
| 2.0 | – | 98.33 ± 1.66a | 7.6 ± 0.4ab | 7 |
| – | 0.5 | 63.34 ± 7.26b | 6.8 ± 0.37bc | 20 |
| – | 1.0 | 51.67 ± 6.0bc | 5.8 ± 0.2c | 18 |
| – | 2.0 | 41.66 ± 3.33c | 4.0 ± 0.32d | 20 |
| 0.5 | 0.2 | 95.0 ± 2.88a | 8.6 ± 0.24a | 10 |
| 0.5 | 0.5 | 88.34 ± 4.4a | 6.8 ± 0.38bc | 15 |
| 0.5 | 1.0 | 18.33 ± 3.3d | 2.6 ± 0.4e | 25 |
Values represent mean ± standard error of 10 replicates per treatment in three repeated experiments. Means sharing by the same letter are not significantly different (P = 0.05) using Duncan’s multiple range test