Table 2.
Detection of seed infection of Tobacco streak virus in seeds collected from sap inoculated and naturally infected groundnut varieties by DAC-ELISA
| Method of seed collection | Variety | Seed infection | A405nm (1 h)b (Range) | |
|---|---|---|---|---|
| Noa. | Percent | |||
| I. Sap inoculated | JL-24 | 40/151 | 26.5 | 0.20–3.00 (0.04–0.09) |
| TMV2 | 42/145 | 28.9 | 0.22–2.00 (0.03–0.05) | |
| Prasuna | 9/34 | 26.5 | 0.23–1.50 (0.04–0.09) | |
| Kadiri 6 | 14/63 | 22.2 | 0.20–1.70 (0.02–0.06) | |
| Kadiri 9 | 7/28 | 25.0 | 0.31–0.50 (0.06–0.08) | |
| Anantha | 11/47 | 23.4 | 0.48–3.00 (0.03–0.09) | |
| Kadiri 7 Bold | 7/37 | 18.9 | 0.55–1.37 (0.03–0.05) | |
| Total | 130/505 | 25.7 | ||
| II. Naturally infected | JL-24 | 27/123 | 21.9 | 0.23–1.80 (0.04–0.06) |
| TMV2 | 31/148 | 20.9 | 0.71–1.51 (0.02–0.06) | |
| Kadiri 6 | 26/136 | 19.1 | 0.23–1.20 (0.04–0.06) | |
| Total | 84/407 | 20.6 | ||
a Number of seeds positive in DAC-ELISA/total number of seeds tested
b DAC-ELISA absorbance values and figures in parentheses were healthy values