. 2012 Sep 19;23(3):303–310. doi: 10.1007/s13337-012-0105-0

Table 1.

Comparison of TCID₅₀/mL amounts resulted from different titration assays

Method of titration
Virus	Traditional^a		Modified traditional^a	MTS^a	MTT^a
Virus	Reed and Muench’s formula	Regression analysis	Modified traditional^a	MTS^a	MTT^a
Cloned EV71	5.8 × 10⁶ ± 2,150,000	5.3 × 10⁶ ± 970,000	5.5 × 10⁴ ± 21000	6.5 × 10³ ± 3400	8.1 × 10³ ± 3700
Clinically isolated EV71	–		4.8 × 10⁴ ± 14000	–	8.7 × 10³ ± 3200

The values are expressed as mean ± SD. The mean are taken from three independent experiments from which standard deviations (SD) were calculated

^aThere was a significant difference with a p < 0.01 between MTT or MTS-based TCID₅₀ and the traditional methods of TCID₅₀. Also, the modified traditional method significantly (p < 0.01) differed from the traditional methods