Pumpkin plant showing leaf curl symptoms in the IARI experimental field. a Amplification of coat protein gene from pumpkin leaf curl sample (lane 1 +ve control ToLCNDV DNA-A clone, lane 2 pumpkin leaf curl, lane 3 negative control, lane M Marker). b Amplification of remaining part (2.0 kb) of the genome of DNA-A with the primers AV 111 and AV 112 from the leaf curl sample (lane 1 pumpkin leaf curl, lane 2 negative control, lane M marker)