Table 1.
Details of nested-PCR and LAMP primers for detection of Hepatitis C virus genomic RNA
| Primer name | Length | Genome position | Sequence | |
|---|---|---|---|---|
| PCR primer | Out-F | 19 | 6–24 | 5′-CTGTGAGGAACTACTGTCT-3′ |
| Out-R | 21 | 282–302 | 5′-GGTGCACGGTCTACGAGACCT-3′ | |
| In-F | 19 | 24–42 | 5′-TTCACGCAGAAAGCGTCTA-3′ | |
| In-R | 21 | 257–277 | 5′-GGGCACTCGCAAGCACCCTAT-3′ | |
| LAMP primer | F3 | 19 | 88–106 | 5′-TCCCGGGAGAGCCATAGTG-3′ |
| B3 | 21 | 254–274 | 5′-CACTCGCAAGCACCCTATCAG-3′ | |
| FIPa | 43 | 148–166 108–127 |
5′-GATCCAAGAAAGGACCCGGTTTTTCTGCGGAACCGGTGAGTAC-3′ | |
| BIPa | 43 | 179–197 232–251 |
5′-CCTGGAGATTTGGGCGTGCTTTTAGTACCACAAGGCCTTTCGC-3′ | |
| Loop F | 17 | 129–145 | 5′-TCATCCTGGCAATTCCG-3′ | |
| Loop B | 18 | 202–222 | 5′-GCGAGACTGCTAGCCGAG-3′ |
aFIP (F1c-TTTT-F2) consists of F2 and F1c sequences as shown on Fig. 1. BIP (B1c-TTTT-B2) consists of B2 and B1c sequences. B2c sequence complementary to B2, F1c sequence complementary to F1