Table 3.
Decimal reduction time* (min) (D-values) of spores of B. cereus cultures
Culture | Heating menstra | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Saline | BHI broth | Skim milk | Whole milk | |||||||||
85 °C | 90 °C | 95 °C | 85 °C | 90 °C | 95 °C | 85 °C | 90 °C | 95 °C | 85 °C | 90 °C | 95 °C | |
B. cereus CFR 1521 | 13.40a | 6.30a | 5.45a | 12.50ab | 7.80a | 5.50a | 11.95a | 6.20a | 5.65a | 9.25a | 7.50a | 4.40a |
(160.8) | (75.6) | (66.0)+ | (150.0) | (93.6) | (62.4) | (144.0) | (74.4) | (68.4) | (111.6) | (90.0) | (52.8) | |
B. cereus CFR 1532 | 14.30a | 6.80a | 5.10a | 12.15b | 9.55a | 4.80a | 15.45a | 12.50b | 7.05a | 9.55a | 6.90a | 4.75a |
(171.6) | (81.6) | (61.2) | (145.2) | (114.0) | (57.6) | (186.0) | (150.0) | (84.0) | (115.2) | (81.6) | (57.6) | |
B. cereus CFR 1534 | 19.45b | 7.90a | 5.90a | 14.30c | 8.30a | 5.35a | 16.45a | 7.70a | 6.10a | 9.15a | 8.75a | 4.95a |
(234.0) | (94.8) | (70.8) | (171.6) | (99.6) | (63.6) | (198.0) | (92.4) | (73.2) | (110.4) | (105.6) | (60.0) | |
B. cereus F 4810 | 19.10b | 6.35a | 5.35a | 13.25a | 7.95a | 5.50a | 12.15a | 7.90a | 5.80a | 10.00a | 7.25a | 5.30a |
(229.2) | (76.8) | (64.8) | (159.6) | (96.0) | (67.2) | (146.4) | (94.8) | (69.6) | (120.0) | (87.6) | (69.6) | |
SEM | ±1.00 | ±0.40 | ±0.31 | ±0.21 | ±0.35 | ±0.18 | ±1.26 | ±0.80 | ±0.53 | ±0.82 | ±0.47 | ±0.59 |
a-cMeans in the same column followed by different superscripts differ significantly (p < 0.05)
*D-values are the means of two experimental trials, with each performed in duplicates
+Figures in parentheses indicate the 12D (commercial sterility) values