Figure A4.

Determination of DGK KO/KD-expression in Arabidopsis T-DNA insertion mutants by RT-PCR. RNA was isolated from 9-days old roots (DGK1,−3,−5,−7) or flowers (DGK2,−4,−6) since the latter genes did not reveal expression in the root (not shown). Predicted band Sizes: DGK1, 702 bp; DGK3, 592 bp; DGK5, 534 bp; DGK6, 434 bp; DGK7, 791 bp; SAND (reference gene), 244 bp. Abbreviations: ND, not detectable; NAC, non-amplification control (test for genomic DNA contamination), i.e., RT reaction without RT enzyme on Col-0 RNA; NTC, no target control (test for contamination + primer dimers), i.e., RT reaction with water sample.