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Kinetics of cold-induced 32P-PtdOH accumulation in Arabidopsis seedlings and leaves. 32P-prelabeled seedlings (A) or leaf disks (B) were incubated at 0°C (filled circles) or 20°C (control, open circles) for different periods of time. Lipids were then extracted, separated by TLC, and quantified by phosphoimaging. Data points (±SD) are from triplicate incubations.
