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. 2013 Jan 22;4:1. doi: 10.3389/fpls.2013.00001

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Copyright © 2013 Arisz, van Wijk, Roels, Zhu, Haring and Munnik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Cold-induced ³²P-PtdOH induction in known Arabidopsis cold response mutants. Five-days-old seedlings were prelabeled O/N with ³²P_i and subsequently incubated at 0°C or kept at room temperature for 15 min. Lipids were then extracted, separated by TLC, and quantified by phosphoimaging. ³²P-PtdOH levels are expressed as percentage of the total ³²P-lipid. Values are means from triplicate incubations from a typical experiment; error bars indicate SD. White bars, control; gray bars, 0°C. (A) The mutants fry1, hos1, los1, and their wt background, C24RD29A-LUC. (B) The snow1 mutant and the wt control, Col-0.