Table 1.
Cold-induced PtdOH formation in seedlings of T-DNA insertion lines.
| Gene | AGI ID | Line | Name | Status* | Control | Cold | Fold increase | Replicates |
|---|---|---|---|---|---|---|---|---|
| wt | Col-0 | wt | 0.98 ± 0.07 | 3.00 ± 0.20 | 3.1 | 6 | ||
| AtDGK1 | At5g07920 | SALK 053412 | dgk1-1 | KD | 0.93 ± 0.07 | 3.11 ± 0.31 | 3.4 | 6 |
| AtDGK2 | At5g63770 | SAIL 718_G03 | dgk2-1 | KO | 1.00 ± 0.06 | 2.70 ± 0.48 | 2.7 | 6 |
| SAIL 71_B03 | dgk2-2 | KO | 0.96 ± 0.10 | 3.19 ± 0.40 | 3.3 | 6 | ||
| AtDGK4 | At2g20900 | SAIL 339_C01 | dgk4-1 | no KO | 1.22 ± 0.08 | 3.73 ± 0.26 | 3.1 | 3 |
| SALK 069158 | dgk4-2 | KO | 0.83 ± 0.08 | 3.65 ± 0.50 | 4.4 | 3 | ||
| AtDGK5 | At2g20900 | SAIL 1212_E10 | dgk5-1 | KO | 0.85 ± 0.07 | 3.73 ± 0.15 | 4.4 | 3 |
| AtDGK6 | At4g28130 | SALK 016285 | dgk6-1 | ND | 1.08 ± 0.03 | 4.48 ± 0.55 | 4.1 | 3 |
| AtDGK7 | At4g30340 | SAIL 51_E04 | dgk7-1 | KD | 0.90 ± 0.17 | 2.92 ± 0.22 | 3.2 | 8 |
| SALK 059060 | dgk7-2 | KD | 0.91 ± 0.14 | 3.24 ± 0.15** | 3.6 | 8 | ||
| SALK 007896 | dgk7-3 | no KO | 0.87 ± 0.14 | 2.94 ± 0.19 | 3.4 | 8 |
5-days-old Arabidopsis seedlings were labeled O/N with 32Pi and incubated at 0°C for 5 min. Lipids was then extracted separated by TLC and quantified by phosphoimaging. PtdOH levels are expressed as a percentage of the total 32P-labeled lipids and values represent averages of multiple samples containing 2 seedlings each (± SD).
*
Transcript analysis by RT-PCR confirmed knock-down (KD) or knock-out (KO) status. Expression of DGK6 was too low for detection hence the status of dgk6-1 could not be determined.
**
Reproducible statistically significant difference of T-DNA line compared with wild type (Tukey HSD test, P < 0.05) within the wild type control homogeneous subset.