Table A2.
Primers used for RT-PCR expression analyses of DGK T-DNA insertion lines.
| Gene | Direction | Primername | Sequence |
|---|---|---|---|
| DGK1 | sense | SALK_053412_LP | GGATTCTCCTCCCGTAGATTG |
| antisense | SALK_053412_RP | TCATGCCGTACTGGAAAATTC | |
| DGK2 | sense | dgk2 fw | GACTGAGAGTTCCACTTTCTC |
| antisense | dgk2 rv | GATCTACTCCACCCATATAGC | |
| DGK3 | sense | SALK_082600_LP (dgk3-1) | TGCTCTCAGTGGGAAGAGATC |
| antisense | dgk3 rv | CAAACTTCATTCCTCACAACAC | |
| DGK4 | sense | dgk4 fw | GCAGTTGTTGCATTGAATCTAC |
| antisense | dgk4 rv (A) | CCAAAGACTGGTGAGGGACTC | |
| antisense | dgk4 r3 (B) | CGCATCTTTCCAGTCTCCTC | |
| DGK5 | sense | dgk5 fw2 | CCAGTGGCAGGACCTCCAC |
| antisense | dgk5 rv | GGAATCTTGAAGGTATCCGCAG | |
| DGK6 | sense | dgk6 fw | CCTGGAACAGATAGGTCTTCG |
| antisense | dgk6 rv | CATTGGCCATTTCTATCACAAATCTG | |
| DGK7 | sense | SALK_007896_LP | CTCCAGGAGTTTTAGTTGGGG |
| antisense | dgk7 rv | GTTGTTTCCATGGTTCACCATCC | |
| SAND ref | sense | At2g28390-Q-fw | CAGACAAGGCGATGGCGATA |
| antisense | At2g28390-Q-rv | GCTTTCTCTCAAGGGTTTCTGGGT |
Sequences of the different primer pairs used to measure the expression of the various DGK genes used in this study. SAND (At2g28390) expression was used as the reference gene (Hong et al., 2010).