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. 2013 Jan 22;3:1103. doi: 10.1038/srep01103

Figure 4. KCl depolarization increases BDNF levels and BDNF antibody blocks KCl-induced sustained ERK activation.

Figure 4

(A) The hippocampal slices were treated with KCl and the level of mature BDNF protein was examined 1 h after the treatment. Sample Western blots of BDNF and GAPDH proteins are shown in (A1) and quantified summary data are shown in (A2; n = 4). KCl treatment increased BDNF protein level. (B) BDNF antibody blocks KCl-induced sustained ERK activation. The activation of ERK was examined in the hippocampal slices 1 h after KCl treatment. The sample blots (B1) and quantified summary data (B2, n = 6) show that the KCl-induced sustained ERK activation was blocked by BDNF antibody. Compared to control, the BDNF antibody alone did not affect basal ERK activation (p>0.91). Asterisks denotes significant difference (p<0.05).