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. Author manuscript; available in PMC: 2013 Jan 22.
Published in final edited form as: J Immunol. 2010 Jan 20;184(4):2148–2155. doi: 10.4049/jimmunol.0902248

FIGURE 6.

FIGURE 6

Collagen secretion by human fetal cardiac fibroblasts exposed to supernatants from macrophages incubated with ICs composed of Ro60-associated ssRNA is FcγRIIIa-dependent. A, Human fetal cardiac fibroblasts were prepared as monolayers and treated using conditions described in Fig. 4. Cells were plated into four-chamber slides (24 h) and serum starved (24 h). B, Cells were incubated with supernatants in the absence or presence of a TNF-α–neutralizing Ab (1 µg/ml) or a TGF-β–neutralizing Ab (1 µg/ml) for 24 h. After the 24-h incubation, supernatants of fibroblast cultures were retrieved and total collagen was detected by the Sircol assay. Results are representative of three experiments. Bars represent means ± SEM.