Fig. 2.

Effects of Miltefosine and Fumonisin B₁ on the proliferation of epimastigote forms of T. cruzi in vitro. Cultures of epimastigotes (5 × 10⁻⁵.mL⁻¹) of Dm28c (A and C) and Y (B and D) strains were prepared at day 0 in the absence (Control) or presence of increasing amounts of Miltefosine (A, B) and Fumonisin B₁ (C, D), and the number of cells was determined daily by direct counting. At days 10 (*) and 19 (*), parasites were collected by centrifugation and adjusted to their starting densities (5 × 10⁻⁵·mL⁻¹) using fresh media containing the same original concentrations of Miltefosine or Fumonisin B₁. Each subculture was followed daily by counting for a total of 32 days as indicated at the bottom of each graph. The results shown are the means ± standard error of two sets of independent experiments.