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Published in final edited form as: Mol Biochem Parasitol. 2011 Dec 30;182(1-2):62–74. doi: 10.1016/j.molbiopara.2011.12.006

Fig. 6 — Rescue of YPK9 lag1Δlac1Δ by overexpression of TcCERS1. (A) YPK9 (wild-type) cells containing the empty histidine-based plasmid p423TEF vector (YPK + vector), YPK9 lag1Δlac1Δ transformed with pBM150:LAG1 (LAG1 in uracil-based plasmid) (2Δ.LAG1), 2Δ.LAG1 containing the empty p423TEF vector (2Δ.LAG1 + vector), and 2Δ.LAG1 containing the p423TEF:TcCERS1 vector (2Δ.TcCERS1) were plated on minimal media containing galactose (SGal) or glucose (SD), amino acids (aa) and FOA (1 mg·mL⁻¹) as indicated beneath each plate. The cells were streaked heavily, and the plates were photographed after 5 days incubation at 30°C. (B) The experiment was performed as in (A), but the transformants were diluted to a final concentration of 1 × 10⁷ cells·mL⁻¹; 3 μL of 10-fold dilutions (from left to right) were spotted onto the plates and incubated for 5 days at 30 °C.

Fig. 6 — Rescue of YPK9 lag1Δlac1Δ by overexpression of TcCERS1. (A) YPK9 (wild-type) cells containing the empty histidine-based plasmid p423TEF vector (YPK + vector), YPK9 lag1Δlac1Δ transformed with pBM150:LAG1 (LAG1 in uracil-based plasmid) (2Δ.LAG1), 2Δ.LAG1 containing the empty p423TEF vector (2Δ.LAG1 + vector), and 2Δ.LAG1 containing the p423TEF:TcCERS1 vector (2Δ.TcCERS1) were plated on minimal media containing galactose (SGal) or glucose (SD), amino acids (aa) and FOA (1 mg·mL⁻¹) as indicated beneath each plate. The cells were streaked heavily, and the plates were photographed after 5 days incubation at 30°C. (B) The experiment was performed as in (A), but the transformants were diluted to a final concentration of 1 × 10⁷ cells·mL⁻¹; 3 μL of 10-fold dilutions (from left to right) were spotted onto the plates and incubated for 5 days at 30 °C.