Skip to main content
. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Biochem Parasitol. 2011 Dec 30;182(1-2):62–74. doi: 10.1016/j.molbiopara.2011.12.006

Fig. 8.

Fig. 8

Metabolic labeling of parental and complemented yeast cells using [3H]myo-inositol. YPK9, YPK9.2Δ.LAG1 (2Δ.LAG1) and YPK9.2Δ.TcCERS1 (2Δ.TcCERS1) growing exponentially on SC were labeled with [3H]myo-inositol as described in item 2.4. Lipids were extracted, deacylated with a mild-base (NaOH) as indicated and analyzed by TLC using chloroform/methanol/H2O (10:10:3, v/v) followed by autoradiography. The major inositol-containing phospholipids (PI, IPC/D and MIPC) typically made by yeast and abnormal lipids (arrows) made by YPK9.2Δ.TcCERS1 are indicated.