Fig. 5.

Fluorine analogs do not alter IL-2 secretion and NFAT expression in Jurkat cells. Jurkat T cells were treated with DFPA or DFA at the concentrations indicated. Additional treatment groups included a vehicle (veh) control and a no treatment control (labeled “cells”). IL-2 secretion by treated and control Jurkat T cells after stimulation with anti-CD3 and anti-CD28 for 24h was measured by standard ELISA techniques. Error bars reflect ± SEM and asterisks (*) indicate statistically significant results, p < 0.05 using ANOVA with a Student-Newman-Keuls post hoc test. NFAT expression in treated and control Jurkat T cells transfected with a NFAT luciferase plasmid was assessed after treatment as previously described. Results are representative of three separate experiments performed in triplicate.