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Proinflammatory cytokine content of untreated PCLS lysates after ~3, 24, 48, and 72h of tissue culture. The tissue slicing procedure induced large increases in IL-1β, CINC, and TNF-α content that subsided by 48–72h of culture. Preparing PCLS caused only a small increase in IL-4, IL-5, IFN-γ, and IL-13 (not shown) content relative to the LLOD of the assays (~1–10 pg/ml).
