Figure 6.

Fabrication and assembly process for microgels via railed microfluidic and acoustic field. (A) Schematic of photolithography methods used to fabricate microgels.⁸⁷ (B) Railed microfluidic assembly of microgels, (a) schematic of a microtrain, (b) assembled 3 × 3 matrix with two different living cells using cell-encapsulating thylene glycol diacrylate solution, and (c) fluorescent images of cell-encapsulating microgels. Scale bar 150 μm.²³ (C) (a) Schematic of acoustic assembly of microgels; (b and c) before and after acoustic excitation, single-layer formation (200 μm × 200 μm microgels, scale bar 200 μm); (d) new microgels were introduced onto a single layer to generate a double-layered structure (scale bar 200 μm); (e) multilayered structure using layer-to-layer approach (scale bar 200 μm).²⁴