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. Author manuscript; available in PMC: 2013 Jan 22.
Published in final edited form as: Methods Mol Biol. 2012;917:219–230. doi: 10.1007/978-1-61779-992-1_13

Figure 1.

Figure 1

Schematic of ΦC31 integrase mediated insertion of plasmid DNA into a chromosome. ΦC31 integrase recognizes and binds to the attB site in the reporter plasmid. Typically the regions to be expressed would be flanked by insulators. Insulators are represented by octagons. The chromosome (double black lines) with a pseudo-attP site is bound in juxtaposition with the attB site in the plasmid. The attB site in the reporter plasmid and a pseudo-attP site in the embryo genome undergo recombination to form hybrid sites attR and attL that flank the integrated reporter plasmid.