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. Author manuscript; available in PMC: 2013 Jan 22.
Published in final edited form as: Methods Mol Biol. 2012;917:219–230. doi: 10.1007/978-1-61779-992-1_13

Figure 3.

Figure 3

Confirming ΦC31 integrase mediated transgenesis and expression. (A) PCR amplification of an endogenous gene (NKX2.10) compared to the amplification of a transgene encoding a T7epitope tagged version of Nkx2-5. 200ng of DNA from individual stage 47(28)embryos was analyzed. Lane 1–8 is the amplification of DNA from eight different embryos using the primers for an endogenous gene, lanes 1′-8′ the amplification of the DNA from the same eight embryos using primers that query the presence of the transgene. Embryos 7 and 8 were transgenic. (B) Analysis of the endogenous expression of Nkx2-5 compared to the transcription controlled by a transgene expressing GFP under the control of the NKX2-5 promoter. Analysis used 200 ng of mRNA isolated from stage 46 embryos and RT-PCR and sampled sequential cycles from cycle 33–36.