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The fluorescence intensity of each mutant was recorded from 290 to 400 nm at an excitation wavelength of 280 nm at 25°C. Fluorescence emission spectra of (A) C72S and the hydrophobic core double mutants at pH 4.0, and (B) at pH 6.0. (C) The salt bridge mutants at pH 4.0, and (D) at pH 6.0. Tables 1 and 2 show the maximum fluorescence intensities and λmax.
