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. Author manuscript; available in PMC: 2013 Jan 22.
Published in final edited form as: Gastroenterology. 2011 Mar 25;141(1):259–268.e8. doi: 10.1053/j.gastro.2011.03.043

Figure 2.

Figure 2

Dkk1d/d mice exhibit increased crypt length during colitis and faster recovery following mucosal injury. (A) Clinical disease activity index and (B) body weight graph of mice subjected to DSS-induced colitis for 7 days, followed by regular drinking water for 7 days. Arrows indicate animals that had to be killed due to severe morbidity. (C) H&E staining revealed comparable disease activity but increased crypt length in Dkk1 hypomorphic mice on day 7. Scale bars = 200 μm. (Di) BrdU incorporation (green) for 1 hour showed increased proliferation in elongated colonic crypts. (Dii) Dkk1d/d mice displayed excessively long crypts in inflamed areas of the distal colon. Scale bars = 100 μm. (E) Morphometric analysis showed a significant crypt lengthening in the entire colon on day 7. Measurements were grouped as mild or severe inflammation, based on histologic appearance. (F) Faster recovery of Dkk1d/d mice was supported by increased colon length at the end of the recovery period. Quantitative data in E and F are derived from 3 to 4 mice per group. *P < .001, **P < .05.