Figure 4.

Reduced AKT/β-catenin signaling in Dkk1^d/d mice during acute colitis. (A) AKT activity and AKT-mediated phosphorylation of β-catenin at residue 552 were determined by immunoblotting. (B) (Upper panels) Reduced AKT activity in Dkk1^d/d mice during colitis was additionally shown by lack of plasma membrane association of AKT phospho-T308 (green). (Lower panels) Localization of β-catenin phospho-S552 (green) revealed patchy staining, particularly at the crypt base, in Dkk1^d/d mice. Arrows indicate cells with nuclear phospho-β-catenin. Scale bars = 100 μm.