Table 1.

Strains and plasmids used in this study

Strain	Plasmid	Promoter	Gene	# if isolates	Description
WT				11	Control
WT pSET152	pSET152			22	Control
WT allN	pDG1	PermE*	allN	28	Over-expression
WT allN+mgl	pDG2	PermE*	allN+mgl	25	Over-expression
WT fkbR	pDG3	PermE*	fkbR	24	Over-expression
WT fkbN	pDG4	PermE*	fkbN	29	Over-expression
ΔallN	pDG5		ΔallN	7	Gene inactivation
ΔallNΔfkbN	pDG5, 8		ΔallN ΔfkbN	2	Gene inactivation
ΔfkbN	pDG8		ΔfkbN	10	Gene inactivation
ΔfkbR	pDG6		ΔfkbR	6	Gene inactivation
ΔfkbRΔfkbN	pDG6, 8		ΔfkbR ΔfkbN	2	Gene inactivation
ΔfkbR	pDG3	PermE*	fkbR	18	Complementation
ΔfkbN	pDG4	PermE*	fkbN	33	Complementation
ΔfkbRΔfkbN	pDG4	PermE*	fkbN	9	Complementation
WT	pSET152		rppA	12	Negative control
ΔfkbR	pMB1	PermE*	rppA	9	Positive control
	pMB2	PallA	rppA	15	Promoter activity
	pMB3	PfkbR	rppA	15	Promoter activity
ΔfkbN	pMB4	PfkbN	rppA	12	Promoter activity
	pMB5	PfkbB	rppA	15	Promoter activity
	pMB6	PfkbG	rppA	15	Promoter activity