Table 1.
Growth of Thermacetogenium phaeum with different substrates in pure and syntrophic co-culture
| Pure culture | ΔOD600 | Cell density [mg · l-1] | Substrate utilized [mM] | Acetate formed [mM] | electron balance [%] | Molar growth yield YE[g · mol-1] |
|---|---|---|---|---|---|---|
| Acetoin |
0.05 |
12.5 |
7.3 |
10.5 |
97.5 |
1.71 |
| 2,3 Butanediol |
0.046 |
11.5 |
4.5 |
8.3 |
151.3 |
2.56 |
| Methanol |
0.583 |
145.8 |
12.2 |
7 |
86.5 |
11.95 |
| Ethanol |
0.047 |
11.75 |
15.3 |
17.2 |
75.5 |
0.77 |
|
Syntrophic culture |
|
|
|
|
|
|
| Acetoin |
0.377 |
94.25 |
7.4 |
12.5 |
128.5 |
12.74 |
| 2,3 Butanediol |
0.045 |
11.25 |
7.7 |
11.4 |
120.1 |
1.46 |
| Methanol |
0.02 |
5 |
0.2 |
0.2 |
- |
- |
| Ethanol | 0.07 | 17.5 | 20.2 | 15.7 | 52.3 | 0.87 |
Molar growth yields (Y) and electron balance were calculated using the formula <C4H7O3> for cell material and the experimentally determined conversion factor OD600 = 1 = 0.250 g cell mass· L-1.
Data show growth after 4 weeks. In most cases no further growth could be observed.