Table 1.
Antibodies
| Primary antibodies (species and type) | Dilution | Supplier |
|---|---|---|
| α-Tubulin (mouse monoclonal) |
1:1000 |
Abcam (Cambridge, MA, USA) |
| Arp2 (rabbit polyclonal) |
1:100 |
Santa Cruz Biotechnology (Santa Cruz, CA, USA) |
| CaM (rabbit monoclonal or mouse monoclonal) |
1:200 |
Abcam |
| Iba-1 (rabbit polyclonal) |
1:1000 |
Wako Chemicals (Richmond, VA, USA) |
| Orai-1 (goat polyclonal) |
1:100 |
Santa Cruz Biotechnology |
| SK3 (rabbit polyclonal) |
1:200 |
Alomone (Jerusalem, Israel) |
| STIM1 (rabbit polyclonal) |
1:100 |
Sigma-Aldrich (St. Louis, MO, USA) |
| Talin1/2 (mouse monoclonal) |
1:100 |
Abcam |
| TRPM7 (goat polyclonal) |
1:200 |
Santa Cruz Biotechnology |
| Vimentin (mouse monoclonal) |
1:1000 |
Millipore (Billerica, MA, USA) |
| Vinculin (mouse monoclonal) |
1:200 |
Sigma-Aldrich |
|
Secondary antibodies | ||
| DyLight 488 or 594 (donkey anti-rabbit) |
1:500 |
Jackson Immunoresearch (West Grove, PA, USA) |
| DyLight 488 or 594 (donkey anti-mouse) |
1:500 |
Jackson Immunoresearch |
| DyLight 488 (donkey anti-goat) | 1:500 | Jackson Immunoresearch |