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. 2012 Dec 27;11:176. doi: 10.1186/1476-511X-11-176

Figure 1.

Figure 1

3T3-L1 cells were induced to differentiate in presence or absence of different concentrations of LI10903F. Intracellular Lipids were stained with Oil Red O. Representative photomicrographs show lipid accumulation in the cells treated with 0.1% DMSO as vehicle control (A); cells treated with 10, 25 and 50μg/ml LI10903F (B, C, and D, respectively). Panel E, bar diagram represents percent inhibition of adipogenesis (mean ± SD) in LI10903F treated 3T3-L1 cells relative to the vehicle control cultures (n=3). Bound Oil Red O dye was eluted from treated adipocytes with 100% isopropyl alcohol, and the OD was read at 550 nm. * Denotes significantly different compared to control cultures, p<0.05. Panel F, 3T3-L1 adipocytes were treated with either different concentrations of the herbal blend (10, 25 or 50μg/mL) or 0.1% DMSO (control). Lipid breakdown was estimated based on the amount of glycerol released in the culture medium. Bar diagram represents released glycerol (mean ± SD) in LI10903F treated and vehicle treated cultures (n=3). *Denotes significantly different compared to vehicle treated controls, p<0.05.