. 2012 Oct 19;12:239. doi: 10.1186/1471-2180-12-239

Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant genotype and/or description	Source or reference
**X. campestrispv. campestris strains**
B100	Wild-type, Sm^r	[46]
B100-6.01	Control strain, carrying ΩKm(cat) in intergenic region flanked by tonB1 and exbB1, Sm^r, Km^r	[64]
B100-5.05	tonB1-deficient mutant, Sm^r, Km^r	[64]
B100-7.03	exbB1-deficient mutant, Sm^r, Km^r	[64]
B100-9.01	exbD1-deficient mutant, Sm^r, Km^r	[64]
B100-11.03	exbD2-deficient mutant, Sm^r, Km^r	[64]
B100-Bac2	Bacitracin-resistant spontaneous mutant of B100, unable to produce polysaccharides, Sm^r	D. Steinmann, CeBiTec culture collection
**E. colistrain**
XL1Blue	recA1, thi, supE44, lac, [F’proAB lacI^q, lacZΔM15, Tn10(Tc^r)]	[89]
Plasmids
pUC6S	lacZα, Ap^r	[90]
pBCKS+	pUC19, lacZ, Cm^r	Stratagene
pBCSK+	pUC19, lacZ, Cm^r	Stratagene
pMS246	pSVB30, aacC1, Gm^r	[91]
pHGW31	pHIP, aacC1ΔBglII, Gm^r	[64]
pHGW241	pHGW31, tonB1, Gm^r	[64]
pHGW242	pHGW31, exbB1, Gm^r	[64]
pHGW243	pHGW31, exbD1, Gm^r	[64]
pHGW244	pHGW31, exbD2, Gm^r	[66]
pIJ3051	pLAFRI-based cosmid carrying 27.9 kb chromosomal BamHI fragment of X. campestris pv. campestris 8004 with pglI, Tc^r	[39]
pHGW260	pHGW31, 11.1 kb chromosomal BamHI fragment of X. campestris pv. campestris 8004 with pglI, Gm^r	This study
pHGW261	pBCKS+, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260, Cm^r	This study
pHGW262	pBCSK+, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260, Cm^r	This study
pHGW267	pUC6S, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260 expressed from aacC1 P_out promoter, Ap^r, Gm^r	This study