Skip to main content
. 2013 Jan 22;8(1):e54485. doi: 10.1371/journal.pone.0054485

Figure 4. Detection of LacZ expression in a Nolz-1 expressing domain in rostral hindbrain of line #22 UREA-LacZ embryos.

Figure 4

A–C: Expressions of β-gal protein (A), LacZ mRNA (B), and Nolz-1 mRNA (C) on coronal hindbrain sections of line#22 E14.5 UREA-LacZ embryo. D–E: High magnification views of the bracketed regions in A–C, respectively. Expressions of LacZ mRNA and β-gal protein are both detected in a differentiated domain of rostral hindbrain (triple arrows in D, E) where endogenous Nolz-1 mRNA is expressed (F). LacZ expression is, however, absent in the nearby Nolz-1-expressing ventricular zone (vz, F). Double arrows in A and B indicate a domain in which β-gal expression is detected with little LacZ mRNA expression. IV, fourth ventricle; mb, midbrain.