Figure 13.

In vitro developing oocytes supplied with organelles from fibroblasts resulting in fibro-oocyte hybrids, or by satellite cells produced by the oocytes and exploited for the progression of the oocyte growth. Images from time lapse photography (time in hours:minutes). A) In vitro developing oocytes (o) deficient in organelles (white arrow) can be joined (arrowhead) by a fibroblast (fb and white arrowhead). B) The optically dense organelles are supplied to the oocyte. C) Alternatively, the oocyte is supplied by adjacent satellite cell (s) with an extended tube (black arrowhead; see detail in inset). D) When completed, the tube disappears (inset) and the satellite is regressing (rs). E) In contrast, the fibroblast moves above the oocyte and releases organelles out (white arrowhead) of the regressing oocyte (ro). F) Subsequently, a fibro-oocyte (fbo) hybrid is formed exhibiting oocyte remnants (ro). Bar in A for A-F. Panels A-D adapted with permission from [9], © Wiley-Liss, Inc, and complemented with E and F.