Figure 7. Electrophysiological characterization of Ca²⁺ currents during embryo development in Styela plicata.

A) Representative traces of voltage protocol and current records obtained in response to voltage steps between −70 and +20 mV, in 10 mV increments, from a holding potential of −80 mV in the developmental stage reported (left). Images (right) at the light microscope of the stages considered. Arrows indicate the polar body. B) Average current-voltage relationship (I/V curve) showing that maximal currents generated at the step to −20 mV. All I/V curves are obtained by averaging the data from twenty considered embryo stages. Error bars indicate S.E.