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. 2013 Jan 22;8(1):e54604. doi: 10.1371/journal.pone.0054604

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© 2013 Gallo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Light shading shows percentage of first cleavage (2-cell stage) of GV-C oocyte fertilized in ASW (control) and in NiCl₂ (a vs b P<0.01). Dark shading shows percentage of 2-cell stage that reached the larval stage. Insert reports a normally developed Styela plicata larva 24 h after fertilization. Bar is 240 µm.