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. 2013 Jan 22;8(1):e54851. doi: 10.1371/journal.pone.0054851

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© 2013 Tokarz et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The measurement of four glucocorticoids in extracts from adult zebrafish holding water was performed by a separate LC-MS/MS analysis of each fraction: (A) unconjugated steroids; (B) glucuronidated steroids; (C) sulfated steroids. Each bar represents the mean value of three independent samples with standard deviations added. Sample abbreviations: X-mixed gender group; F-all female group; M-all male group; X-E-mixed gender group, without addition of digestive enzymes; N-water control without fish.