Table 1. Primer sequences for quantitative real-time PCR.
gene name | accession number (Ensembl) | primer sequences (5′ 3′) | amplicon length (bp) | |
actb1 | ENSDART00000055194 | for | AAGGCCAACAGGGAAAAGAT | 110 |
rev | GTGGTACGACCGGAGGCATAC | |||
eef1a1l1 | ENSDART00000023156 | for | CAAGGAAGTCAGCGCATACA | 189 |
rev | GCATCAAGGGCATCAAGAAG | |||
rpl8 | ENSDART00000140039 | for | CCCCTTTCGCTTCCTCTTT | 185 |
rev | GTCCTTCACGATTCCCTTGA | |||
hsd20b2 | ENSDART00000100769 | for | AATGGTTGAAAGGGGGAAAG | 201 |
rev | TTATGGGTCATGTTCGTGGA | |||
hsd11b2 | ENSDART00000141211 | for | GGGGGTCAAAGTTTCCACTA | 165 |
rev | TGGAAGAGCTCCTTGGTCTC | |||
cyp11c1 | ENSDART00000061572 | for | ATGAAGTGGCGCAGGATTT | 215 |
rev | CTCCACAGCCGAAATGAAG | |||
star | ENSDART00000016225 | for | AACAAGGGCAAGAAGCTCTG | 207 |
rev | CCCCCATTTGTTCCATGTTA | |||
crh | ENSDART00000038290 | for | TCTGTTGGAGGGGAAAGTTG | 198 |
rev | ATTTTGCGGTTGCTGTGAG | |||
mc2r | ENSDART00000077231 | for | CTCCGTTCTCCCTTCATCTG | 126 |
rev | ATTGCCGGATCAATAACAGC |