Figure 2. Prevention of ribosome assembly on HCV IRES by SLRef RNA. (A) Representation of Sucrose gradient sedimentation profiles of α ³²P UTP-labeled HCV 5′ UTR RNA in the presence or absence of 200 fold molar excess of either SLRef RNA or SLRf RNA. Percentage CPM represents the percentage of total counts added to the reaction against the fraction number of gradient. The fractions were collected from top downwards. The 80S and 48S peaks were indicated. (B) Representation of sucrose gradient sedimentation profiles of α ³²P UTP-labeled capped Luc RNA in the presence or absence of 200 fold molar excess of SLRef RNA. (C) Sucrose gradient sedimentation profiles of α ³²P UTP-labeled HCV 5′ UTR RNA incubated in the RRL, in the presence of 2 mM GMP-PNP or 2 mM of cycloheximide. (D) Representation of sucrose gradient sedimentation profiles of α ³²P UTP-labeled HCV 5′ UTR RNA in the presence or absence of 200 fold molar excess of mSLRef RNA