Table 1. Sequence information of fzd examined.

Name of gene	Sequence of forward primer	Sequence of reverse primer	Enzyme for probe	Accession No.
fzd1	5′-AGC TCT GCG TGG GAC AGA AC	5′-ACG AAA GCT GAG CTT CAC AC	PstI/T7	BC163358
fzd2	5′-CGC ATC CGA ACC ATC ATG AA	5′-AGC AAA TGA GAG AGA GTG GC	PstI/T7	NM_131140
fzd3a	5′-ATG AGCAGA GCC ATA GAT CG	5′-ACC CCA GAC TTC TGT TGG TA	NcoI/SP6	NM_001042761
fzd3b	5′-ACT GTG ACG AGT CTT ACC CT	5′-TTA CGC ACT GGT CCC GTT CT	NcoI/SP6	AB246777
fzd4	5′-TAA ACT GCA GCC TTT TCC CG	5′-CAC AAC CGT CTC GTT TCC TT	NdeI/T7	XM_002664725
fzd5	5′-TGC ATG GAC AGA AAC AGC AG	5′-TCA GAC ATG TGA TGA GGG TG	SpeI/T7	AF117387
fzd6	5′-TAA ATG GAT TAA GCT CCG CC	5′-GAC AGA AAC TCT GAG TTT AGA G	SalI/T7	NM_200561
fzd7a	5′-CAG AAC CAT CAT GAA GCA CG	5′-CAG CGA AAC TGT ACA GAT AC	SpeI/T7	AF336123
fzd8a	5′-AGG AGT GTC ATT AAG CAG GG	5′-GCT TTT CGT GTG GCA TCT AC	NcoI/SP6	AF039412
fzd9a	5′-GTA TTC CTG CAG TGA AGA CC	5′-GTT TTA TTG CTC TTC CTT TGA G	SalI/T7	NC_007116
fzd9b	5′-CCC ACC AGA AAT GAC CCT AA	5′-TCA TAC ATG TGT GGG ACA GT	SpeI/T7	AF169639
fzd10	5′-CGT AAG GTC ATG AAG ACC GA	5′-AAC CAG GGA CAT GAT CTCAC	NcoI/SP6	AF039411