Table 4. Melanisation occurs in sox10 mutant heterozygous embryos injected with fzd3a, 9b, 10-MO at 1 dpf.

Amount of MO	Elongated axis+ no/slight necrosis	Elongated axis+ severe necrosis	Shortened axis+ severe necrosis	Dead	Total
standard-MO 20 ng	37 (37)	0 (0)	0 (0)	0	37
fzd3a-MO 10 ng	48 (48)	0 (0)	0 (0)	1	48
fzd9b-MO 15 ng	49 (49)	0 (0)	0 (0)	1	50
fzd10-MO 5 ng	30 (30)	14 (13)	0 (0)	1	45
fzd3a, 9b, 10-MO  = 10, 5, 2 ng	22 (22)	19 (17)	1 (0)	0	42
= 10, 2, 2 ng	22 (22)	15 (15)	0 (0)	3	40

Notes.

50% of embryos examined for each category should be sox10 mutant heterozygotes. Numbers in parenthesis are of significantly melanised embryos. See Table 3 for definition of categories.