Figure 5. Gene expression of Sp_0149 during infection.

(A) Ethidium stained agarose gel showing equal amplification efficiency for the primer pairs used for PCR of psaA and metQ when using S. pneumoniae 0100993 genomic DNA as the template. (B) Ethidium stained agarose gel of cDNA products generated by RT-PCR after 26 and 30 cycles using S. pneumoniae 0100993 total RNA extracted from the blood of infected mice and primers for 16S rRNA (internal control), psaA (positive control) and metQ. (C) Relative mRNA concentrations of selected genes of S. pneumoniae WCH43 (serotype 4) in various in vivo niches at 72 h post-intranasal infection of mice. Transcript abundance for each gene was obtained by microarray analysis, and normalized against those obtained for the 16S rRNA control. Quantitative fold differences for each transcript were determined as a ratio of its abundance to that of the 16S rRNA control. Data are means ± SEM for each gene transcript from three replicate challenge experiments.