Figure 9. Analysis of the surface expressions of known HCV (co)receptors in 420Bla+ stable cells.

(A and B) Huh7 cells were transfected with JFH1 or 420Bla RNAs, and cultured in the absence (−) or presence (+) of blasticidin for 3 days. Huh7 cells indicate the mock-transfected cells cultured in parallel. The cells were harvested for analyzing protein expressions (A) and surface expressions of HCV (co)receptors and CD59 by flow cytometry (B). (C) A set of representative results from three independent CellQuest flow cytometric analyses from (B) was shown as histograms. The purple shaded area, green solid line, pink dashed line, blue dashed line, and red solid line indicate the IgG isotype control, Huh7 cells, JFH1, 420Bla−, and 420Bla+ cells, respectively. Data represents mean ± SEM (n = 3).