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(A) The γ-lactone-forming reaction catalyzed by mouse SMP30/GNL. The product of the catalytic reaction of SMP30/GNL is l-gulono-γ-lactone, which is in turn converted to ascorbic acid by gluconolactone oxidase. (B–D) Substrate and product analogues used in this study: (B) xylitol, (C) 1,5-anhydro-d-glucitol (1,5-AG), and (D) d-glucose. Corresponding atoms in these molecules are marked in light orange and light yellow.
