Table 2. Comparison of STK295900 with camptothecin, etoposide, and Hoechst 33342 for its effect on the proliferation of various cancer and non-cancer cell lines.

		IC50 (µM)
Cell Line	STK295900	Camptothecin	Etoposide	Hoechst 33342
HeLa	0.64	0.02	0.30	4.56
MCF7	0.04	0.03	13.21	0.20
HepG2	0.14	0.02	0.84	0.22
HT-29	0.21	0.03	10.26	0.65
hTERT RPE-1	3.43	<0.01	0.45	1.01
267B1	1.61	<0.01	0.39	0.84
MRC5CV1	0.95	0.02	1.29	0.35

Cells were seeded at 1−2×10³ cells in 96 well plates and treated with various concentrations of STK295900, camptothecin, etoposide, or Hoechst 33342. Cell growth was determined by MTT assay for up to 4 days. All experiments were done at least in triplicates, and IC₅₀ was calculated from dose-response curves.