Figure 8. Carbon monoxide or heme oxygenase-regulated chlorophyll accumulation under Fe-deficiency was correlated with NO action.

Cells were grown in the iron-sufficient Fe (10 µM) nutrient solution for 5 days, and then transferred to iron-limited Fe (0.1 µM) media with or without 8 µM CO for 8 days. Meanwhile, the CO-exposed cells were treated with 3 µM cPTIO. After that, (a): the cell were loaded with 15 µM 4,5-diaminofluo-rescence (DAF-2DA) for 15 min and immediately photographed (bar = 1 mm). (b): the cell phenotype was photographed. (c): the gene transcript levels. 18S was used for cDNA normalization. The number below the band indicates the relative abundance (RA) of the genes with respect to the loading control 18S.